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Standard slice recording methods were used to obtain Fig. 1. Briefly, patch clamp recordings were obtained under visual guidance using infrared optics from tex2html_wrap_inline1083m slices from Long Evans rats (P14-P20). Recordings were performed at 33-35C. Slices were continuously perfused with a solution containing (in mM) NaCl, 120; KCl, 3.5; CaCltex2html_wrap_inline1085, 2.6; MgCltex2html_wrap_inline1085, 1.3; NaHtex2html_wrap_inline1085POtex2html_wrap_inline1091, 1.25; NaHCOtex2html_wrap_inline1093, 26; glucose, 10, which was bubbled with 95% tex2html_wrap_inline1095 and 5% tex2html_wrap_inline1097, and the pH of which had been adjusted to 7.35. All recordings were obtained in the presence of the AMPA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX; tex2html_wrap_inline1099). Recording pipettes were filled with (in mM) K gluconate, 170; HEPES, 10; NaCl, 10; MgCltex2html_wrap_inline1085, 2; EGTA, 1.33; CaCltex2html_wrap_inline1085, 0.133; MgATP, 3.5; GTP, 1.0 (pH 7.2). Resistance to bath was 3-5 Mtex2html_wrap_inline1107 before seal formation.

Data were acquired using a National Instruments (Texas) AT-MIO-16-F-5 A/D card on a Pentium-based computer under the Window NT (Microsoft) operating system. Software written in Labview (National Instruments) with Dynamic Data Exchange links to Matlab (Mathworks) allowed convenient online synthesis and injection of arbitrary synthetic current waveforms.

Tony Zador
Fri Nov 28 10:17:14 PST 1997