Standard slice recording methods were used to obtain Fig. 1. Briefly, patch clamp recordings were obtained under visual guidance using infrared optics from m slices from Long Evans rats (P14-P20). Recordings were performed at 33-35°C. Slices were continuously perfused with a solution containing (in mM) NaCl, 120; KCl, 3.5; CaCl, 2.6; MgCl, 1.3; NaHPO, 1.25; NaHCO, 26; glucose, 10, which was bubbled with 95% and 5% , and the pH of which had been adjusted to 7.35. All recordings were obtained in the presence of the AMPA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX; ). Recording pipettes were filled with (in mM) K gluconate, 170; HEPES, 10; NaCl, 10; MgCl, 2; EGTA, 1.33; CaCl, 0.133; MgATP, 3.5; GTP, 1.0 (pH 7.2). Resistance to bath was 3-5 M before seal formation.
Data were acquired using a National Instruments (Texas) AT-MIO-16-F-5 A/D card on a Pentium-based computer under the Window NT (Microsoft) operating system. Software written in Labview (National Instruments) with Dynamic Data Exchange links to Matlab (Mathworks) allowed convenient online synthesis and injection of arbitrary synthetic current waveforms.